 |
|||
 |
|||
 |
|||
 |
 |
Proteomics Facility Core
Surface Enhanced Laser Desorption Ionization ToF-MS (SELDI) 1. Description: This is a unique approach to mass-spec in which proteins are separated on the target itself by virtue of their chromatographic properties. The ability to retain proteins on the chip and wash away impurities and proteins that interfere with ionization allows direct analysis of protein profiles from body fluids and tissue lysates. 2. Application: Biomarker identification Protein expression profiling 3. Limitations: The proteins are not identified. The information the Investigator receives is that a protein of a given mass is more or less abundant Requires large number of samples (>30 per group is recommended) for confidence that a true change in abundance of any protein exist between groups Although there is some ability to quantify the amount of any protein with the SELDI system, mass-spec is not a quantitative technique and the quantitation should be viewed as relative and confirmed by another technique if possible 4. Advantages: Provides a quantitative (see limitations above) measure of changes in the abundance for hundreds of proteins in a sample The chromatographic steps for isolation of the protein of interest are determined from the characteristics of binding to the different SELDI surfaces 5. Equipment: Ciphergen Mass Spectrometer 6. Services: Consultation on uses of SELDI Training in use of SELDI chips and the mass-spec |
|
 |
|||
 |
|||
 |
|||
 |
|||
 |
|||
 |
|||
 |
|||
 |
|||
 |
|||
 |
|||
 |
|||
 |
|||
 |
|||