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Imaging and Cytometry Facility Core Core Leader: Kamiar Moin, Ph.D. Associate Core Leader: Xiaoxin Susan Xu, Ph.D. Associate Core Leader: John J. Reiners, Jr., Ph.D. The purpose of the Imaging and Cytometry Facility Core (ICFC) is to enhance the research activities of EHS Center members by providing access to contemporary technology, instrumentation, and services in confocal, fluorescent and light microscopy and flow cytometry. The ICFC also provides consultation services to investigators who would like to introduce microscopy or flow cytometry applications into their research programs. It is a primary goal of the Core to facilitate preliminary as well as ongoing research projects among EHS Center members. For additional information, contact Dr. Kamiar Moin
Services: ICFC services and instrumentation include:
Equipment: Specialized equipment and instrumentation available for use includes: Flow Cytometry: A Becton Dickinson FACSCalibur is available for Center member use. This instrument is equipped with two lasers: a standard Argon laser @ 488 nm and a red diode laser @ 635 nm. The additional laser allows analysis of up to four fluorochromes simultaneously in addition to forward scatter (cell size) and side scatter (granularity, complexity). The FACSCalibur is controlled by a FACStation which consists of a Macintosh Power PC 7600 loaded with Becton Dickinson Immunocytometry Systems acquisition and analysis software including CellQuest, ModFit, FACSComp, FACSConvert, Attractors, and Paint-A-Gate. The FACSCalibur was purchased with electronics, fluidics and a cell concentrator module for cell sorting. Center members can make appointments to use the flow cytometry facility by calling Kim Zukowski (313-963-4322). Confocal
Microscopy: A Zeiss 310 Laser Scanning Confocal Microscope is
available for use. This microscope is equipped with three lasers: a
543 nm HENE, a 488 and 514 nm multiline Argon, and a 350 nm
multiline Argon. This allows investigators the ability to label
their samples with up to three fluorophores. This instrument is able
to analyze two fluorophores simultaneously and the third fluorophore
within seconds. In addition to the confocal microscope, a Zeiss
Axiophot dual camera fluorescent photomicroscope, identical to the
one integrated in the confocal setup but without the lasers or the
confocal optics, is housed in the same laboratory. Researchers may
preview their slides and/or take 35mm photographs of their samples
from this microscope. This setup allows at least two individuals
access to a fluorescent microscope at all times. Center members can
make appointments to use the Confocal Microscopy branch of the ICFC
by contacting Dr. Kamiar Moin (313-577-2511).
Immunocytochemistry and Immunohistochemistry: The ICFC performs comet assays and immunocytochemistry for Center Members. The Core has several fluorescent microscopes including a Nikon Microphot that has both halogen and epifluorescent light sources and a 35mm camera for documentation. Also available is a Nikon Optiphot equipped with halogen and epifluorescent light sources, a ConSensus black and white digital camera for quantitative fluorescence, a Spot color digital camera, a filter wheel with filters for dapi, acridine orange/Texas Red, FITC, FITC/Texas red/dapi, and a computer with MetaMorph software for image analysis. Center members can make appointments for immunohistochemistry or microscope usage by calling Kim Zukowski (313-963-4322). Three-Dimensional Reconstruction of Confocal Images and Computer Graphics: The equipment and expertise is available in the ICFC to output any microscopic images generated within the Core for either routine documentation, or for publication. The confocal microscope facility will assist in reconstructing multiple serial images and adjusting various parameters into a three dimensional image. Dr. Moin offers expert advice on the best way to represent complicated 3-D images. Center members can make appointments to use the graphics services of the Confocal Microscopy branch of the ICFC by contacting Dr. Kamiar Moin (313-577-2511). Sample Analysis and Data Acquisition Instrumentation: The ICFC also maintains equipment and instrumentation for sample analysis and data acquisition (e.g., microplate spectrophotometer and fluorometer, IS4400 gel documentation system, x-ray film processor, laser densitometer). Contact Dr. Xiaoxin Susan Xu (313-577-0100) for more information. |
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