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Cell Culture and Gene
Transfer Technologies Facility
Core
Core Leader:
Thomas
A. Kocarek, Ph.D.
The mission of the
Cell Culture Facility Core (CCGTTFC) is to support the goals of the EHS Center
Research Cores by providing their investigators with the cell
culture systems, methodologies, equipment and technical assistance
that will enable them to address important questions in the
environmental health sciences, with human emphasis. This mission is
accomplished by providing:
-
physical space,
equipment and supplies for performing cell culture work
-
cell culture
services that meet the needs of the Center Research Core members
-
technical
assistance, education and training The Cell Culture Facility
Core is located in the Institute of Environmental Health
Sciences, and features fully-equipped cell culture laboratories
that are available for investigator use.
For additional
information, contact
Dr. Thomas Kocarek.
Services:
The Cell Culture
Facility Core offers services that (1) help investigators place
cells into culture, (2) help investigators perform their own cell
culture experiments, (3) improve the consistency and quality of cell
cultures and (4) enhance the abilities of investigators to study
biological processes in mechanistic detail.
The Cell Culture
Facility Core currently offers the following services:
- Isolation and
primary culture of animal hepatocytes and Kupffer cells
- Distribution
and primary culture of human hepatocytes
- Preparation and
culture of precision-cut tissue slices
- Acquisition,
maintenance in culture, cryopreservation and distribution to
users of cell lines (please inquire for information about cell
lines that are currently available through the Core)
- Isolation of
cells in different stages of the cell cycle by centrifugal
elutriation
- Transfection
support services Preparation of recombinant adenoviruses
-
Preparation/distribution of culture medium
- Testing of cell
lines for mycoplasma contamination
- Provision of
space, equipment and sterile supplies for cell culture work
- Provision of
training, assistance and consultation in cell culture techniques
Equipment:
Specialized equipment
that is available for use includes:
-
Centrifugal
elutriation rotor
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Tissue slicer and
dynamic organ incubator
-
Computerized image
archiving system for storing microscopic images
-
Dedicated
laboratory for preparing and using recombinant adenoviruses.
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